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Download Advances in Tracer Methodology: Volume 3 A collection of by Sterling Chaykin (auth.), Seymour Rothchild (eds.) PDF

By Sterling Chaykin (auth.), Seymour Rothchild (eds.)

ISBN-10: 146848625X

ISBN-13: 9781468486254

ISBN-10: 1468486276

ISBN-13: 9781468486278

Radioactive tracers have had a profound influence on clinical examine in past times two decades, quite in bio­ chemistry and allied fields. The speedy evolution of subtle instrumentation and labeling options in the past ten years has made attainable on a regimen foundation exquisitly delicate experiments which with different recommendations will require her­ culean efforts at the a part of the investigator. The Symposia on Advances in Tracer technique, that have been held frequently because 1957, supply for examine employees in biochemistry, pharmacology, endocrinology, and drugs a spotlight at the a variety of advancements within the tracer box which are frequently scattered in the course of the clinical literature. quantity three is arecord of the complaints of the 9th and 10th Symposia, that have been subsidized by way of the hot England Nuclear company and the Packard device corporation, Inc. fresh advances in tracer technique, as mirrored by way of the papers during this quantity, were normally excited about im­ proved analytical strategies, akin to liquid scintillation count number­ ing, autoradiography, and chromatography, and with novel ap­ plications of classified compounds in biochemical and scientific problems.

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Additional info for Advances in Tracer Methodology: Volume 3 A collection of papers presented at the Ninth and Tenth Symposia on Tracer Methology

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Pat. 610,426 (May 17, 1962). A. 57 P 16482a (i962). 8. 0 .. and Warne, R. I•• ]. Chern. Soe. 1893 (1951). SYNTHESIS OF 35S_p_TOLUENE SULFONIC ANHYDRIDE (TOSAN) OF HIGH SPECIFIC ACTIVITY L. Thuneberg Institute of Experimental Medicine Copenhagen, Denmark This paper describes the synthesis of 35S-p-toluene sulfonic anhydride of high specific activity, the details of which have been published elsewhere [1], and thoroughly covers some recent improvements in technique, together with present experience concerning the use of the 35S-labeled reagent in the analysis of aldosterone in peripheral plasma.

1944). 36 Preparation of Labeled Compounds 8. Plattner, P. , Ruzicka. , Helv. Chim. Acta 30:1073 (1947). 9. , Z. Physio1. Chem. 296:87 (1954). 10. Geiling, E. M. , Kelsey, F. , McIntosh, B. , and Ganz, A.. Science 108:558 (1948). 11. ,]. Pharmacol. Exp. Therap. 110:244 (1954). 12. , Second International Symposium on the Chemistry of Natural Products, Prague (August 27 to September 2, 1962). 13. , Monatsber. Deut. Akad. Wiss. Berlin 5:299 (1963). 14. , Chem. Ber. 95:2557 (1962). 15. Straub, W•• Biochem.

From these data it is obvious that a high degree of purity of the 35S-labeled reagent is an absolute requirement when a high esterification yield and a low blank value are desired in the aldosterone analysis. On the basis of this, we found that an increased initial purity of the 35S-tosan was desirable, in order to increase the stability during storage, or, alternatively, the 35S-reagent should not be stored in anhydride form, but rather as the free acid, since the latter, on account of its greater chemical stability, might also be less sensitive to irradiation damage.

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Advances in Tracer Methodology: Volume 3 A collection of papers presented at the Ninth and Tenth Symposia on Tracer Methology by Sterling Chaykin (auth.), Seymour Rothchild (eds.)

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