By R. K. Salyaev (auth.), Dr. Bernard P. Marin (eds.)
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Additional info for Biochemistry and Function of Vacuolar Adenosine-Triphosphatase in Fungi and Plants
Several osmium-iodide mixtures are quickly reduced at the exoplasmic surface of the vacuole membrane but not at the protoplasmic surface (Marty 1973; Butor and Marty 1984). The staining pattern seen at the electron microscope is suggestive of an internal vacuolar skeleton stemming from the membrane. Its chemical composition remains unknown but polyanions could contribute. These observations reveal the unique microenvironment at the exoplasmic surface of the vacuole membrane. Finally, colloidal gold particles coated with ConA have been incubated with unsealed fragments derived from the vacuole membrane of red beetroot.
Fourty perc e nt of the total integral membrane proteins ar e prefer e ntially partitioned with the ' exoplasmic leafl e t of the lip id bilayer and occupy 16 % of the vacuole membrane area (Table 4). As computed from the shadowed replicas, many of these integral prot e ins could span the bilayer. The background matrix is made moderate ly rough by patches of small depressions. The complementary protoplasmic fracture (PF) face contains particles which are more dens e ly packed than those of the EF fac e (Table 4).
23 A B A 75K 73K 59K 50K 59K 50K B c o 42K 33K 33K 27K 27K 17K 12K 17K 12K Fig. 7. 5 %). (A) Membrane polypeptides recovered in the pellet. 5 % Triton X-lOa Fig. 8. Identification of glycoproteins in the vacuole membrane. Gels stained with Coomassie Blue (A), with FITC-ConA (B), with FITCWGA (C), or with FITC-RCA (D) Carbohydrate-containing polypeptides from the vacuole membrane of red beetroot have been detected in SDS-gels using PAS reagent and FITClabeled lectins of defined specificity (Marty and Branton 1980).
Biochemistry and Function of Vacuolar Adenosine-Triphosphatase in Fungi and Plants by R. K. Salyaev (auth.), Dr. Bernard P. Marin (eds.)